DNA hydrogel networks stem cell-specific trapping efficiency

Recently, the Institute of Chemical Engineering and Technology Yang Dayong research group studies on DNA hydrogel network capture of stem cells on the American Chemical Society published in the Journal. School of Chemical Engineering and Technology, Dr. Yao Han Tang pool and graduate students as co-first authors. Study was funded by the National Natural Science Foundation. Related achievements China has applied for patents for inventions. Stem cells are pluripotent cells with a class of self-replication, wherein the mesenchymal stem cells have unique biological functions, such as homing to the site of the damaged tissue differentiate into various cell types, secreting various biologically active molecules and immunomodulatory function, great value for biomedical research and cellular therapy. Mesenchymal stem cells in bone marrow content is very low, only 0.001% to 0.01% of the total mononuclear cells, therefore, thousands of bone marrow cells from stem cells to capture and maintain the activity of stem cells challenging. Deoxyribonucleic acid (DNA) as molecular genetic life, is programmable biological macromolecules, in recent years new beginning for the manufacture of biological materials, particularly DNA flourish hydrogel material (Progress in Polymer Science, 2019, 101163). Recently, the Institute of Chemical Engineering and Technology, Professor Yang Dayong research group has developed a strategy based on the captured cell DNA hydrogel network of physical crosslinking, the realization of bone marrow mesenchymal stem cells efficiently capture three-dimensional envelope and enzymatic release. This strategy cell capture is achieved in two long DNA strand synergy: DNA strand 1, inclusive, can be efficiently targeted to the aptamer sequence of dry cells, stem cell-specific anchor; DNA strand comprising the DNA strand -1 -2 portion complementary sequence, three-dimensional network is formed by the -1 strand DNA hybridization, stem cells to achieve a three-dimensional encapsulated; under action of nucleases, DNA is degraded network, controlled release of stem cells. Such physically crosslinked hydrogel network DNA cell culture not only provides an elastic, semi-moist and three-dimensional extracellular matrix environment class, and which facilitates the discharge of the appropriate pore size and metabolic waste transport nutrients cell ensures encapsulated cells were maintained good activity. In this study based on the use of programmable and molecular recognition of DNA molecules, DNA network may be designed with different structures and functions, there is provided a common strategy to capture other types of cells and culture.

Figure 1. stem cells designed for capturing DNA hydrogel network. A) by Rolling Circle Amplification methods (RCA) long strand DNA synthesis, and a schematic view of a three-dimensional network through the DNA base pairing between complementary DNA strands. B) DNA molecules in the process of forming the diffusion and phase transformation network. DNA strand DNA strand 1 and -2, respectively and SYBR Green II Gel Red staining. C) Capture efficiency, encapsulated and enzymatically released dimensional schematic of: 1) Capture: DNA strand 1 and the bone marrow mesenchymal stem cells (of BMSC) incubation aptamer Apt19S anchor BMSC; 2) enclosing: introduction of DNA strand -2 to trigger the formation of a DNA network, the three-dimensional encapsulated BMSC; 3) release: DNA network may be DNase I nuclease degradation, release BMSC.

Paper information: Chi Yao +, Han Tang +, Weijian Wu, Jianpu Tang, Weiwei Guo, Dan Luo, Dayong Yang *, Double Rolling Circle Amplification Generates Physically Crosslinked DNA Network for Stem Cell Fishing Journal of the American Chemical. Society, 2020. original link: https: //pubs.acs.org/doi/10.1021/jacs.9b11001