Hong Kong Branch academician Zhong Tang this team and professor of Soochow University attached to a hospital HOU whole team: the near-infrared region II-induced aggregation of fluorescent probes to achieve intraoperative imaging ureter

iatrogenic ureteral injuries (IUIs, iatrogenic uretral injuries) is one of the most serious complication of abdominal and pelvic surgery, gynecologic tumors incidence of up to 10%, often cause fistula, urinary pus toxemia, or even chronic renal insufficiency. In addition, the need to rebuild the ureter of UPJO related diseases such as, the ureter often due to inaccurate positioning of the lesion, causing excessive surgery operation, causing secondary damage to the ureter. Therefore, real-time recognition of ureteral surgery for the treatment of prevention of iatrogenic ureteral injury ureteral and related diseases is essential. Current clinical techniques used to identify ureter spontaneous ureteral peristalsis, Kelly\’s sign, stand pipe, intravenous injection of methylene blue. However, their effectiveness is limited ureteral injury prevention, therefore, to develop an effective method for ureteral identification is necessary during surgery. II near infrared region (NIR-II, at a wavelength of 1000-1700 nm) of the optical imaging because of its high resolution imaging and tissue penetration depth, in terms of navigation technique show great potential. Recently, Professor TANG Zhong academicians team and the First Affiliated Hospital of Soochow University, Hong Kong University of Science and Technology HOU whole team reported a nanoparticle near infrared region II 2TT-oC6B dots, rabbits realized intraoperative ureteral fluorescence imaging . 2TT-oC6B dots having AIE molecules (AIEgen) and approved by the FDA DSPE-PEG2000 housing configuration (FIG. 1). An emission peak at 1030 nm, the fluorescence quantum yield as high as 11.0%.

FIG 1. 2TT-oC6B dots synthesis scheme. After

First, the fluorescence imaging dots and indocyanine green (ICG) was compared 2TT-oC6B, injected retrograde ureter lumen 2TT-oC6B dots, real-time NIR-II was observed by the fluorescence signal to the ureter and creep, and the ureter 2TT-oC6B dots fluorescence imaging signal to noise ratio (SBR) and the depth of penetration is far better than ICG.

[1232TT-oC6B dots (left) and the developing region II near infrared fluorescent contrast] [123 within] 2. FIG ureter the ICG (right). 香港科大唐本忠院士团队与苏州大学附一院侯建全教授团队合作:近红外II区聚集诱导发光的荧光探针实现术中输尿管成像
established iatrogenic ureteral injury (FIG. 3), ureter disease model (FIG. 4), after injection of retrograde ureter 2TT-oC6B dots, by NIR-II fluorescence signals are accurately positioned ureteral injury or the site of the disease. In addition, the situation after recanalization ureter patch may also be assessed (FIG. 5) by injection 2TT-oC6B dots.
Figure 3. The near infrared fluorescence imaging region II iatrogenic ureteral injuries. 香港科大唐本忠院士团队与苏州大学附一院侯建全教授团队合作:近红外II区聚集诱导发光的荧光探针实现术中输尿管成像
Figure 4. ureter disease common region II near infrared fluorescence imaging. 香港科大唐本忠院士团队与苏州大学附一院侯建全教授团队合作:近红外II区聚集诱导发光的荧光探针实现术中输尿管成像
Figure 5. ureteral injury repair region II near infrared fluorescence imaging 香港科大唐本忠院士团队与苏州大学附一院侯建全教授团队合作:近红外II区聚集诱导发光的荧光探针实现术中输尿管成像
Accordingly, we have developed high in stability, NIR-II fluorescence signal strong AIE nanoparticles 2TT-oC6B dots, animals achieved intraoperative ureteral identification, and provides a new strategy for the real-time fluorescence imaging in clinical surgery. More relevant results were published in \”

ACS Applied Materials & Interfaces \” (ACS Appl.Mater Inter.2020, 12 (7):. 8040-8049), the Impact Factor: 8.456. The first author of the paper to Suzhou University doctoral student Du Jian , co-first author Hong Kong University of Science and Technology Postdoctoral Liu Shunjie , Chinese Academy of Sciences Shenzhen Institute of Advanced Technology Pengfei researcher [123 ], corresponding author for the Hong Kong University of Science and Technology TANG Zhong academicians and the first Affiliated hospital of Soochow University, full Professor Hou, Zhang Weijie physician . Paper information:. Du J, Liu S, Zhang P, et al Highly Stable and Bright NIR-II AIE Dots for Intraoperative Identification of Ureter [J] ACS Applied Materials & Interfaces, 2020, 12 (7):. 8040-8049 Link:. https: //doi.org/10.1021/acsami.9b22957