Huang Xiaojun, Zhejiang University / Hangzhou Normal University School of Medicine, Chen Jing, Xie Tian cooperation: new findings on cell membrane gradient sensing

Glucose is the basic cell growth energy source, directly involved in cell metabolism oxidation of adenosine triphosphate (ATP). Metabolic pathways of tumor cells different from normal cells, even in the case of sufficient oxygen tumor cells are more inclined metabolized by the glycolytic pathway, resulting in a large number of tumor cells consume glucose and other nutrients in the surrounding environment. Monitoring of tumor metabolic processes in-depth understanding of the mechanism of tumor growth, one of the important methods of drug inhibition, provide a new entry point for the study of traditional cell biological methods. How parameters continuously cultured in situ detection of tumor cells in a continuous metabolic microenvironment of the biosensor prepared presents a great challenge. This team bioelectrochemical gradient sensor system based on the polysulfone hollow fiber membrane and a cell culture assembly constructed in situ in one set of electrochemical detection. First, the inner wall of the hollow fiber membrane layer assembly nanotubes – Prussian blue – composite electrochemical sensor structure of the enzyme, followed by culturing adherent cells in a denser outer wall of the hollow fiber membranes. Their internal and external surfaces of the hollow fiber membranes and electrochemical detection cell growth to achieve zonal isolation, to avoid direct contact with the electrode cell, reduce the impact on the current cell growth, while metabolites achieve balanced internal and external environment through the porous membrane wall, in order to achieve long-term electrochemical monitor. The porous structure enhances the stability of the enzyme electrode, and the consumption of H2O2 produced by introducing a catalytic reaction layer Prussian blue, reduce long-term monitoring of the accumulation of H2O2. Construction of cell culture with glucose detection system by monitoring the actual dosing lung cancer cell growth, initially confirmed the feasibility of glucose in the membrane-electrode cell metabolism and cell culture environment of continuous detection substance, it will further be applied to drug screening and cell Metabolism. related to the results of \”In-situ monitoring of glucose metabolism in cancer cell microenvironment based on hollow fiber structure\” was published journal Biosensors and Bioelectronics (IF = 9.518) at a high level. The first author of the paper Hangzhou Normal University College of Pharmacy graduate Ma Zhen; thesis advisor Professor Chen Jing Hangzhou Normal University School of Medicine, Zhejiang University Professor Xie Tian and highMolecular Science and Engineering Associate Professor Huang Xiaojun. This work was funded by the National Natural Science Foundation of China, Zhejiang Provincial Natural Science Foundation and other projects. Papers link: https: //doi.org/10.1016/j.bios.2020.112261