Injectable hydrogel may promote healing of infected wounds

Protein is a key component of all tissues, due to its superior biocompatibility and low immunogenicity, has been widely used in tissue engineering. However, the lack of natural proteins typically vascularization, bone and neural differentiation inducing activity function further limits its use as a functional biomaterials. In addition, due to the complexity of its structure, easy to inactivation, denaturation, etc., resulting in its modification difficult. Therefore, it is necessary to find a new method specific functionality modified to feature-rich protein-based biomaterials, so that the functional protein-based biomaterials to get promotion, design and application. Polypeptide having antimicrobial, wound healing, anti-tumor effect. However, the modified polypeptide is currently mostly confined to the surface of biomaterials, modification of 3D repairing tissue defects, there are still difficulties. Therefore, 3D polypeptide biological material with a certain activity is expected to function for the design of peptide biomaterials open up new ideas. Recently, , Ruijin Hospital, Shanghai Jiaotong University School of Medicine / Orthopedics Shanghai Institute of injury Liu Zhihong, Cuiwen Guo, Tang Lian-fu and others According to bovine serum characteristics of the extracellular matrix such cells albumin (BSA), by thiolated BSA, KK polypeptide Ag + and synergistic crosslinking successfully constructed polypeptide protein injectable hydrogel has antibacterial and vascularization for facilitating infection wound healing. where, S-Ag hydrogel dynamic interactions impart unique rapid degradation, injectable, self-healing, antibacterial and angiogenesis of . The 3D multifunctional polypeptide protein hydrogels have broad application prospects wound healing in infection biomedical field. Related outcomes to \”Injectable Polypeptide-Protein Hydrogels for Promoting Infected Wound Healing\” was published in the journal Advanced Functional Materials. 可注射水凝胶促进感染伤口的愈合

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1. Preparation process of KK-BSA gel

First, Traut\’s reagent to give BSA BSA-SH. BSA-SH was then mixed with AgNO3 and KK-SH, S-Ag ligand by crosslinking in situHydrogel forming protein polypeptide (KK-BSA gel).

可注射水凝胶促进感染伤口的愈合
Preparation 1 KK-BSA gel and FIG role in the process of wound repair infected FIG. A) with BSA-SH, KK-SH and Ag + S-Ag constructed dynamic coordination hydrogel. B) KK-BSA hydrogel in situ repair infected wounds dynamic process: infection while promoting wound healing, promoting the continuous release of antimicrobial Ag + KK polypeptide and neovascularization. C) injecting the hydrogel wound infection, promote wound healing.

2. Characterization of KK-BSA gel

and KK-BSA BSA gel gel has a uniform pore structure. After mixing KK polypeptide and BSA having excellent flowability, was added after the AgNO3 mixed to form a gel having a certain strength. The results show that KK-BSA and injectable gel having self-healing properties.

可注射水凝胶促进感染伤口的愈合
Characterization of morphology and hydrogel FIG. A) Chemical Formula KK-BSA and BSA synthesis gel. B, C) Scanning electron micrographs of KK-BSA and BSA gel. D, E) the gelation process KK-BSA hydrogel. F, G) of the injectable gel photograph. H-K) KK-BSA gel self-repair property. O) a schematic view of self-repairing mechanism: S-Ag dynamic reversible key.

3. In vitro effect of KK-BSA gel

Cell experiments show, KK-BSA gel has good biocompatibility, S-Ag solution by a double bond and from Ag + release played a good antibacterial effect. Further, KK polypeptide can increase the expression level of VEGF to promote angiogenesis.

可注射水凝胶促进感染伤口的愈合
FIG. 3 A) control, BSA KK-BSA group and group 3, and 5 days live / dead fluorescence results. B) HUVECs group, BSA group and KK-BSA group forming ability in vitro vessel for 12h. C) BSA and KK-BSA gel antimicrobial susceptibility of Staphylococcus aureus, 12h, and 24h for the agar diffusion experiments. D) in the different groups 1, 3 and quantitative results of CCK-8 for 5 days. E) the diameter of zone of inhibition KK-BSA and BSA group S. aureus. F) group, BSA group, and KK-BSA group, the total length of the vessel at 12h.

4. vivo effect KK-BSA gel

Since the early massive release of silver ions and KK peptides, BSA and KK-BSA hydrogel group on day 7 were higher than heal in the control group. As the duration of treatment, KK-BSA group continued to increase the area of ​​wound healing, on the 14th day of wound healing rate of 95.8 ± 1.2%, exhibits excellent effect to promote healing of infected wounds and antibacterial.

可注射水凝胶促进感染伤口的愈合
FIG 4 infection in vivo wound healing. A) The first 0,3,7,10 and 14 days to accept the different treatment of the wound tissue Photos. B) different sets of wound healing in vivo boundaries. Yellow skin tension zone represents the initial wound area, the blue area indicates the area of ​​the wound 3,7,10 and 14 days. C) a different set of wound healing rates at each time point.

The full text link: https: //onlinelibrary.wiley.com/doi/epdf/10.1002/adfm.202001196

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