Injectable hydrogel may promote healing of infected wounds
Protein is a key component of all tissues, due to its superior biocompatibility and low immunogenicity, has been widely used in tissue engineering. However, the lack of natural proteins typically vascularization, bone and neural differentiation inducing activity function further limits its use as a functional biomaterials. In addition, due to the complexity of its structure, easy to inactivation, denaturation, etc., resulting in its modification difficult. Therefore, it is necessary to find a new method specific functionality modified to feature-rich protein-based biomaterials, so that the functional protein-based biomaterials to get promotion, design and application. Polypeptide having antimicrobial, wound healing, anti-tumor effect. However, the modified polypeptide is currently mostly confined to the surface of biomaterials, modification of 3D repairing tissue defects, there are still difficulties. Therefore, 3D polypeptide biological material with a certain activity is expected to function for the design of peptide biomaterials open up new ideas. Recently, , Ruijin Hospital, Shanghai Jiaotong University School of Medicine / Orthopedics Shanghai Institute of injury Liu Zhihong, Cuiwen Guo, Tang Lian-fu and others According to bovine serum characteristics of the extracellular matrix such cells albumin (BSA), by thiolated BSA, KK polypeptide Ag + and synergistic crosslinking successfully constructed polypeptide protein injectable hydrohas antibacterial and vascularization for facilitating infection wound healing. where, S-Ag hydrogel dynamic interactions impart unique rapid degradation, injectable, self-healing, antibacterial and angiogenesis of . The 3D multifunctional polypeptide protein hydrogels have broad application prospects wound healing in infection biomedical field. Related outcomes to \”Injectable Polypeptide-Protein Hydrogels for Promoting Infected Wound Healing\” was published in the journal Advanced Functional Materials.
1. Preparation process of KK-BSA gel
First, Traut\’s reagent to give BSA BSA-SH. BSA-SH was then mixed with AgNO3 and KK-SH, S-Ag ligand by crosslinking in situHydrogel forming protein polypeptide (KK-BSA gel).
2. Characterization of KK-BSA gel
and KK-BSA BSA gel gel has a uniform pore structure. After mixing KK polypeptide and BSA having excellent flowability, was added after the AgNO3 mixed to form a gel having a certain strength. The results show that KK-BSA and injectable gel having self-healing properties.
3. In vitro effect of KK-BSA gel
Cell experiments show, KK-BSA gel has good biocompatibility, S-Ag solution by a double bond and from Ag + release played a good antibacterial effect. Further, KK polypeptide can increase the expression level of VEGF to promote angiogenesis.
4. vivo effect KK-BSA gel
Since the early massive release of silver ions and KK peptides, BSA and KK-BSA hydrogel group on day 7 were higher than heal in the control group. As the duration of treatment, KK-BSA group continued to increase the area of wound healing, on the 14th day of wound healing rate of 95.8 ± 1.2%, exhibits excellent effect to promote healing of infected wounds and antibacterial.
The full text link: https: //onlinelibrary.wiley.com/doi/epdf/10.1002/adfm.202001196