Micro-bar code technology is a versatile technology that provides high-throughput and multiplex information is stored as micro- and nano-scale application of biology, medicine and materials science. However, the current barcode technology mainly using fluorescence spectroscopy and fluorescence intensity multiplexing (FI) coding, they are generally susceptible to spectral coding elements overlap. Further, since the concentration of the sample and the external microenvironment variability, it is difficult to obtain quantitative readings. Using a fluorescence lifetime (fluorescence lifetime), may be reproduced to provide an output in the micro-bar code region. With the latest imaging microscopy, fluorescence lifetime may be used as a simple technique to minimize the limitations of conventional fluorescent bar code, over time and provides repeatable quantitative readings. However, the response of the material to find the flexibility to adjust the fluorescence lifetime remains a continuing challenge.
Based on the above issue, Professor Rachel K. O\’Reilly research group from the UK College of Chemistry, University of Birmingham, designed a light generated by the two components can be switched (photo-switchable) nano gel, the gel exhibited variable fluorescence lifetime by energy transfer induced photoisomerisation caused by light irradiation process. Fluorescence lifetime imaging microscopy may be used (FLIM) visually rendering the fluorescence lifetime characteristics of such manipulation remote, which can selectively store and display information on a microscale. And, in the organelles of imaging, fluorescence lifetime imaging may be live cells and subcellular organelles minimize the influence of background. Related outcomes to \”Manipulating the fluorescence lifetime at the sub-cellular via scale photo-switchable barcoding\” in the title, published in \”Nature communciation\”.
1. Design and fabrication of the optical switch Nanogel
2. Evaluation of energy transfer in the process of photoisomerization and photophysical behavior
3. The optical stimulus reversible fluorescence lifetime
4. Reversible living cells fluorescence lifetime bar code
Herein, researchers have developed a remotely controlling polymer gel nano FRET fluorescence lifetime by one kind of process-induced photoisomerisation. These nanogels system is further adapted to use bar code scanning FLIM fluorescence lifetime, so as to achieve a controllable, reversible and non-invasive method of object tracking and polymorphic fluorescence visualized selectively. As a reading system capable of selectively nanogel quantitative results decoded by the simultaneous extraction of life, thereby allowingXu microscale store information. As a proof of concept, by clicking on the mitochondrial tracer introduced into the reaction nanogels, thereby obtaining a living cell lifespan barcode subcellular size, improved imaging sensitivity. Article link: