Swiss researchers synthesized a new crown virus, capable of producing large amounts of live virus or modified within a week

瑞士学者人工合成出新冠病毒,能在一周之内生产或改造出大量活病毒 new crown pneumonia (COVID-19) epidemic is still spreading worldwide, the World Health Organization (WHO) has listed it as a global pandemic (Pandemic), this is the first time coronavirus infection is evaluated as a global pandemic, even in the 2003 SARS have never reached this level. Up to now (2020, May 5), the total confirmed cases worldwide over 3.6 million cases, a total of 250,000 cases of deaths, nine countries have confirmed a total of over 10 million people, and even the United States has more than 120 million people worldwide within the scope of the epidemic situation is still very grim. The new crown is global epidemic of pneumonia, therefore, the vaccine against the new virus crown, and crown a new cure for the virus, is particularly important, these two types of studies are needed to obtain a new crown virus live virus . May 4, Switzerland University of Bern of Jorerg Jores and Volker Thiel team Nature published a title It is: Rapid reconstruction of SARS-CoV-2 using research papers of a synthetic genomics platform. The study demonstrated based on the full functionality of synthetic genomics platform of yeast, can be within the synthetic gene fragment week on the novel coronavirus (SARS-CoV-2) engineered and resurrection [ 123]. Without isolation of the virus from the patient directly using yeast to quickly produce a large number of active novel coronavirus (SARS-CoV-2), this technology can respond quickly to sudden outbreak of the virus outbreak, you can during an outbreak generate real-time evolving RNA virus variants and characterized its function, significantly reduced due to the difficulties caused by the virus mutates to bring the study . Population growth, increased travel, as well as factors such as climate change, exacerbated by the spread and prevalence of RNA viruses. Only in the last decade, we have witnessed 瑞士学者人工合成出新冠病毒,能在一周之内生产或改造出大量活病毒 Middle East respiratory syndrome coronavirus (MERS-CoV), Zika virus (ZIKV), Ebola virus (Ebola) outbreak, by the end of 2019, Wuhan, Hubei, the first time the novel coronavirus [123 ] (SARS-CoV-2) caused by pneumonia epidemic, the epidemic spread quickly, and soon spread to most Chinese provinces, as well as a number of overseas countries. Chinese scientists have long divided the sort of new active virus crown, but direct share has limitations from the patient or a virus isolated in practice, live virus for diagnosis of disease, animal model, the intermediate host confirmed, vaccine research and development to critical. However, to construct novel coronavirus (SARS-CoV-2) by chemical synthesis, can bypass this limitation, without isolation from the patient\’s body, there can be obtained virus activity , and functional characterization and genetic modification of a single gene can also be a virus. Today, trans to genetics (Reverse genetics) has become an essential tool, it completely changed our views on the virus pathogenesis and vaccine development. However, for example, Coronavirus such a large RNA virus, since the size of the gene set of questions, are difficult to manipulate and cloned in E. coli , thus requiring another fast and powerful anti to study the genetics platform. Accordingly, researchers another way, new antisense DNA viral genome crown broken down into 12 having a length of 0.5-3.4 kbp overlapping terminal sequences 瑞士学者人工合成出新冠病毒,能在一周之内生产或改造出大量活病毒 DNA fragment was added and the green fluorescent protein (GFP) sequence, the detection and observation experiments facilitate subsequent infected cells. Homologous recombination system of S. cerevisiae, such that the antisense DNA in yeast reassembled into a complete gene sequence. Then, in vitro with T7 RNA polymerase transcription of the antisense DNA has become infectious viral RNA. After RNA introduced by electroporation perforations, normally we found to be infected Vero E6 cells and fluorescence. In addition, the research team demonstrated the full functionality based on synthetic genomics platform of yeast, it can be used for more thanKind of rapid reconstruction of many RNA viruses, including members of the 瑞士学者人工合成出新冠病毒,能在一周之内生产或改造出大量活病毒 coronavirus family 瑞士学者人工合成出新冠病毒,能在一周之内生产或改造出大量活病毒, Flaviviridae and paramyxovirus family of. meaning 瑞士学者人工合成出新冠病毒,能在一周之内生产或改造出大量活病毒 study

The research and development of the reconstruction of large RNA virus of the system based on synthetic genomics to quickly synthesize a large number of active novel coronavirus [ 123] (SARS-CoV-2), as well as other RNA viruses, the system does not require clinical samples to provide active virus samples to the health authorities and laboratories. With the

novel coronavirus (SARS-CoV-2) outbreak, the virus may be genetic sequence variation and phenotypic changes occur, use of this system, these mutations can be introduced quickly, and real-time characterization New coronavirus (SARS-CoV-2) function. Overall, the research and development of this new system, breaking the limitations of viral origin, can be mass-produced in a short time (one week) or modified live virus, for use in disease diagnosis, animal model, the intermediate host confirmed vaccine research and development research. More importantly, a strong variation in the ability of RNA viruses, new viruses crown as a large RNA virus, there is also the risk of mutation, this new technology can quickly build a synthetic variation of the virus, and real-time characterization of the epidemic and make real-time changes a more rapid response. Papers link: https://www.nature.com/articles/s41586-020-2294-9

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