The research shows that hydrogen peroxide (H2O2) is one of the molecular diffusion of cancer cells freely, is considered important for understanding important physiological and pathological cells in cell signaling molecule. Wherein the normal level of H2O2 physiological balance between the production and catabolism. However, H2O2 levels in cancer cells significantly increased, H2O2 viable cells to diffuse from the cell membrane. Therefore, real-time monitoring of live cells to release H2O2 is important to understand the behavior and heterogeneity between tumor cells. However, currently the quantitative and distinguishing living cells released from H2O2 is also challenging because it has a fast diffusion, and diluted to a natural decomposition characteristics of ultralow concentration in the extracellular microenvironment. Although the use of electrochemical, colorimetric analysis and chemiluminescence method or the like can be detected by H2O2, but for better biocompatibility, more simple signal probe prepared, the diffusion distance is shorter and less background interference detection techniques need further studies. Based on this, Tsinghua University Professor LIN Jin-Ming and the National Center for Nanoscience of Lin Ling (co-author) jointly reported [ 123] a liquid crystal functionalized elastomeric microspheres (LCEM-HRP) using horseradish peroxidase (the HRP), which may be directly fixed on the cell membrane, thereby releasing the single cell level real-time monitoring of H2O2 [123 ]. LCEM-HRP can be reported to the radial H2O2 (CR) deformed by the same center, which is due to the catalytic reduction of H2O2 by HRP in LCEM-HRP caused by deprotonation and medium chain or between intrachain hydrogen bond breaking LCEM-HRP due. Wherein the level of deformation LCEM-HRP show different amounts of H2O2 released from the cell. In the detection time of 10 min, fixed on the estimated detection sensitivity LCEM-HRP cells was ~2.2 × 10 -7 μM. In addition, researchers explored the heterogeneity of cell lines and cells from a different configuration of LCEM-HRP. In short, LCEM-HRP released from the living cells by in situ monitoring of real-time imaging is provided a new method for H2O2, and may find a basis for more advanced cellular microenvironment chemical probes of various signaling molecules imaged. The research results entitled \”Monitoring H2O2 on the Surface of Single Cells with Liquid Crystal Elastomer Microspheres \”published in internationally renowned journals Angew. Chem. Int. Ed. on. [Graphic Explanation]
first, researchers dissolving liquid crystal diacrylate (2-methyl-1,4-phenylene bis (4 – (((acryloyloxy) hexyl) oxy) benzoate ester) (RM82), E7 7- suberic acid (LC) in (7OAc) monomers and azobisisobutyronitrile (AIBN) synthesis of about 2 μm and natural radial configuration LCEM diameter. Next, horseradish peroxidase (HRP) liquid crystal elastomeric microspheres (LCEM) chemically modified form LCEM-HRP, and is directly fixed on the cell membrane to release the real-time monitoring of single living cells from H2O2. LCEM and Similarly, LCEM-HRP solution at pH = 7.4 (de-ionized water, DIW), and cell culture medium (MEM) exhibit radial configuration. If HRP (HRPrhd) by binding a fluorescent dye to the rhodamine-labeled LCEM ( LCEM-HRPrhd on), it may be determined HRP immobilized on LCEM.
Summing described later, LCEM-HRP provides a reliable method of H2O2 in situ detection in real-time imaging of single cell resolution released from living cells. LCEM-HRP explored the same heterogeneity between different cell surface of tumor cell lines. LCEM-HRP endocytosis does not occur, and therefore can be easily fixed on the cell membrane, having a high biocompatibility, low operating costs, high sensitivity and shorter analysis time (10 min). Further, LCEM-HRP can be the basis for the pursuit of more advanced chemical molecular probes to monitor the various imaging signals from living cells. Document Link: Monitoring H 2 O 2 on the Surface of Single Cells with Liquid Crystal Elastomer Microspheres (… Angew Chem Int Ed, 2020, DOI:. 10.1002 / anie.202004326)